- Breast Cancer
- Oxidative stress
- Ion channels
- Calcium signaling
- BA/BSc (Ibn Zohr University, Agadir, Morocco
- MA/MSc (Universite' de Picardie Jules Verne, Amiens, Picardie, France)
- PhD (Universite' de Picardie Jules Verne, Amiens, Picardie, France)
- PDF (Dalhousie University)
My research interests focus on understanding the potential association between ion channels and oxidative stress in breast and gastric carcinogenesis. The main objective of our research is to find new early-detection tools and develop novel therapeutic strategies to increase the outcome and survival of patients.
Furthermore, we have a particular interest in the dynamic and conformational rearrangement occurring during ion channel gating. Specifically, we are interested in the study of the parts of ion channel that form the pathway through which ion crosses the membrane.
El Hiani Y, Negoda A, Linsdell P. Cytoplasmic pathway followed by chloride ions to enter the CFTR channel pore. Cell Mol Life Sci. 2015 Dec 13. [Epub ahead of print]
El Hiani Y, Linsdell P. Functional Architecture of the Cytoplasmic Entrance to the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Pore. J BIol Chem. 2015 Jun 19; 290(25):15855-65. doi: 10.1074/jbc.M115.656181
El Hiani Y, Linsdell P. Metal bridges illuminate transmembrane domain movements during gating of the cystic fibrosis transmembrane conductance regulator chloride channel. J Biol Chem. 2014 Oct 10; 289(41):28149-59. doi: 10.1074/jbc. M114.593103
El Hiani Y, Ahidouch A, Lehen'Kyi V, Hague F, Gouilluex F, Mentaverri R, Kamel S, Lassoued K, Brule' G, Ouadid-Ahidouch H. Extracellular signal-regulated kinases 1 and 2 and TRPC1 channels are required for calcium-sensing receptor stimulated MCF-7 breast cancer cell proliferation. Cell Physiol Biochem. 2009, 23(4-6):335-46. doi" 10.1159/000218179
El Hiani Y, Lehen'kyi V, Ouadid-Ahidouch H, Ahidouch A. Activation of the calcium-sensing receptor by high calcium induced breast cancer cell proliferation and TRPC1 cation channel over-expression potentially throught EGFR pathways. Arch Biochem Biophys. 2209 Jun 1; 486(1):58-63. doi: 10.1016/j.abb.2009.03.010.
- Mammalian cell culture and transfection
- Patch-clamp electrophysiology: whole-cell, inside out (single channel and macroscopic current recording)
- Calcium imaging
- Molecular Biology (site-directed mutagenesis, qPCR, Western Blot, ELISA)
- Cellular Biology (proliferation, migration and cell cylce progression)