Biological Mass Spectrometry Core Facility

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Pricing

Pricing

Effective date: September 5, 2013

Below are a few examples of different analyses performed in the lab. These prices should be considered as reference values. The exact price will vary according to the analytical and bioinformatic requirements for each sample. A writen quote/estimate may be provided by email upon request.

Note:

  • Academic Rates: +25%
  • External Clients: +50%

Intact Protein Analysis

  User Fee $25
LC-MS/MS MS on Velos Pro*1 $50 direct infusion
Data Analysis / Bioinformatics
Intact MW*11 $50 per Request*2
  Examples
Total for 1 sample
Total for 2 samples
Total for 5 samples
$125
$175
$325

In-gel digestion - Protein ID

  User Fee $25
Sample prep Reduction-Alkylation-Trypsin digestion-Desalting $50 for 1st sample
+ $25 for each additional sample
LC-MS/MS LC-MS/MS on Velos Pro*1 $50 per Sample
Data Analysis / Bioinformatics Protein ID*4 $50 per Request*2
  Examples
Total for 1 sample
Total for 2 samples
Total for 5 samples
Total for 10 samples
$175
$250
$475
$850

In-solution digestion - Protein ID

  User Fee $25
Sample prep Reduction-Alkylation-Trypsin digestion-Desalting $125 per Sample*3
LC-MS/MS LC-MS/MS on Velos Pro*1 $50 per Sample
Data Analysis / Bioinformatics Protein ID*4 $50 per Request*2
  Examples
Total for 1 sample
Total for 2 samples
Total for 5 samples
$250
$425
$950

Label-Free Differential MS Analysis*7

  User Fee $25
Sample prep Reduction-Alkylation-Trypsin digestion-Desalting $125 per Sample*3
LC-MS/MS LC-MS/MS on Velos Pro*1 $50 per Sample
Data Analysis / Bioinformatics Protein ID*4 $50 per Request*2
Protein Quant*6 (Label Free) $100 per Request*2
  Examples
Total for 2 samples
$525

Differential MS Analysis using dimethyl labeling*7

  User Fee $25
Sample prep Reduction-Alkylation-Trypsin digestion-Desalting $125 per Sample*3
Binary peptide Dimethyl labeling $200
Ion exchange separation by FPLC (30 fractions) $300
LC-MS/MS LC-MS/MS on Velos Pro *1 $50 per fraction (x30)
Data Analysis / Bioinformatics Protein ID *4 $100 per Request*2
Protein Quant *5 (Dimethyl labeling) $200 per Request*2
  Examples
Total for 2 samples
$2575

Targeted MRM*8

  User Fee $25
Sample prep Reduction-Alkylation-Trypsin digestion-Desalting $125 per Sample*3
LC-MS/MS LC-MS/MS on Qtrap 5500 $50 per injection*10
Data Analysis / Bioinformatics MRM Quant *9 $150*9
  Examples
Total for 1 sample
Total for 2 samples
$350 (One injection)
$500 (One inj/sample)

Use of LC-MS/MS for Method Development*12

 

Use of LC-MS/MS for Method Development*12

  Qtrap 5500 w/Selexion VelosPRO Orbitrap
Per day $150 $175
Per week (5 days) $700 $800

 

IMPORTANT NOTICE. This table represents a general guideline for most common analyses. Please contact the Facility Lab Manager to inquire about particular or custom-made methods.

 

Discounts

All users (Dal, Academic Rates and External Clients) will receive discounts for  bulk analyses on every LC-MSM injection as follows:

Injection No Discount
1-50
 Regular Price
51-100 10%
101-150 20%
151-200 30%

 

* Notes

*1 Operated on Information Dependant Acquisition (IDA) Mode
*2 Includes all samples submitted on Sample Submission Request Form.
*3 For approximately 100ug of protein in samples
*4 Protein Identification using Proteome Discoverer-Sequest/Mascot search engine.
*5 Protein Quantification using Proteome Discoverer-Sequest/Mascot search engine.
*6 Protein Quantification on Sieve program.
*7 Minimum of two samples (eg. Control-Experimental) required.
*8 Targeted MRM on Qtrap 5500 with Selexion Differential Ion Mobility Source.
*9 Protein Quantification using Multiquant/Skyline data analysis software includes MRM method development and tuning.
*10 Number of injections depends on number of Proteins/Peptides/Transitions being monitored.
*11 Intact protein molecular mass by ESI-MS and spectral deconvolution.
*12 Includes support from staff to operate instrumentation in the lab and basic consumables (solvents, chemicals etc). Requires user to bring their own LC columns. After Method Development, individual sample analysis will be charged according to Proteomics Core Facility Price List for Dalhousie Users (See Table above).