Training & Facility Access
CMDI has been offering skilled hands on training to faculty, students, and staff since 1998.
Are you interested in receiving training and access to the facility? Contact us to book an appointment.
Have you already received training? Login to the online booking calendar to view equipment availability.
https://www.med.dal.ca/medix/index.cfm
Please refer to the facility guidelines before doing so
Tip for Imaging
Be sure to check the excitation/emission spectra of fluorophores before imaging using the https://www.fpbase.org/spectra/
The Cellular & Molecular Digital Imaging Facility has state of the art equipment for biological light microscopy imaging. The equipment available ranges from widefield fluorescence microscopes to live cell super-resolution confocal microscopes all with the latest imaging software.
Browse through the following list and expand any item to view equipment details and technical specifications.
Confocal Microscopes
Zeiss LSM 710 (upright) laser-scanning microscope
Lenses:
10x Plan APOCHROMAT
20x Plan APOCHROMAT
40X oil Plan NEOFLUAR
63x oil Plan APOCHROMAT
100x oil α Plan APOCHROMAT
Zeiss LSM 880 with Airyscan-super resolution imaging technology
Lenses:
10x Plan APOCHROMAT
20x Plan APOCHROMAT
40x oil Plan APOCHROMAT
63x oil Plan APOCHROMAT
100x oil α Plan APOCHROMAT
Leica TCS SP8 near super-resolution setup (lightning) with 4 solid state lasers
Lenses:
10x HC Plan APOCHROMAT
20x HC Plan APOCHROMAT
40x oil Imm HC Plan APOCHROMAT
63x oil Imm HC Plan APOCHROMAT
100x oil Imm HC Plan APOCHROMAT
Lasers: Solid State
405nm
488nm
552nm
638 nm
Filtersets:
DAPI
DAPI/FITC/TXRED
GFP
RHOD_LP
Leica SPI STED super resolution setup with white light laser, 3 depletion lasers, & FLIM capabilities
Lenses:
10x
20x
40x
63x glycerine
93x
100x
Lasers:
White light laser [~440-790]
3 Depletion lasers
GFP
RFP
Far red
Filtersets:
DAPI
DAPI/FITC/TXRED
GFP
RHOD_LP
Zeiss Cell Observer spinning-disk confocal microscope with FRAP & TIRF
| Laser Lines (nm) |
| 405 |
| 488 |
| 561 |
| 639 |
Filtersets:
49 DAPI
38 HeGFP
77 HeGFP/mRFP
Lenses:
10x EC Plan NEOFLUAR
20x Plan APOCHROMAT
40x oil Plan APOCHROMAT
63x oil Plan APOCHROMAT
100x oil alpha Plan APOCHROMAT
Widefield Fluorescent Microscopes
Widefield Fluorescent Microscopes
Zeiss Axio Imager Z1 with Colour and Monochrome Camera
Lenses:
5x Plan NEOFLUAR
10x EC Plan NEOFLUAR
20x Plan APOCHROMAT
40x Oil EC Plan NEOFLUAR
63x Oil Plan APOCHROMAT
100x Oil Plan APOCHROMAT
Filtersets:
49 DAPI
38 He GFP
43 He DsRed
50 Cy5
Zeiss Axio Observer with Monochrome Camera
Lenses:
5x PlanNEOFLUAR
10x PlanNEOFLUAR
20x EC Plan NEOFLUAR
40x LD ACHROMAT PLAN
63x oil Plan NEOFLUAR
100x oil Plan APOCHROMAT
Filtersets:
90 HE DAPI/GFP/CY3/CY5
110 HE DAPI/GFP/CY3.5/CY7
Zeiss PALM laser microdissection
PALM MicroBeam uses a focused laser beam to cut out and isolate selected specimen.
Isolation of sensitive stem cell specimens can occur influencing their vitality or genetic structure.
Laser:
365nm (cutting/catapult laser)
Lenses:
5x FLUAR
10x FLUAR
20x LD Plan-NEOFLUAR
40x LD Plan-NEOFLUAR
63x oil Plan APOCHROMAT
100x oil EC Plan-NEOFLUAR
Filtersets:
38 HE GFP
43 HE DsRed
49 DAPI
50 Cy5
Zeiss Lightsheet Z.1
The Zeiss Lightsheet Z.1 lets you image large cleared specimens with light efficiency and minimal photo damage. Multiple tiles of Z-stacks with several thousand high quality images can be acquired within minutes.
| Lasers | Laser Lines (nm) |
| Laser 405-20 | 405 |
| Laser 488-30 | 514 |
| Laser 561-20 | 561 |
| Laser 638-75 | 638 |
Lenses:
5x
10x
20x
Bruker Skyscan 1276 MicroCT
The SkyScan 1276 is an in vivo micro-CT with continuously variable magnification and low dose imaging for scanning laboratory animals (ie-mice, rats) and other biological samples.
- 20-100 kV X-ray source
- Cooled X-ray camera
- Smallest pixel size 2.8 micron
- 80mm scanning diameter, >300mm scanning length
- Shortest scanning cycle of 3.9 sec
- Physiological monitoring (breathing, movement detection, ECG)
- On-screen dose meter
Computer Workstations
- Mac & PC
- Imaris Workstation version 9.7.0
- Arivis Workstation