Equipment & Services
Next Generation Sequencing
Our facility uses Illumina short read sequencing platforms. We operate an Illumina NextSeq 2000, and NovaSeq X Plus.
We offer library preparation and sequencing services for a range of Illumina sequencing applications.
For more details see the Illumina tab above.
Bioinformatics
We now offer bioinformatics services using the Illumina DRAGEN platform.
Illumina DRAGEN has a number of tools for secondary analysis, more details can be found here.
10X Genomics Single Cell Sequencing
Our 10X Chromium iX instruments are now available for single cell RNAseq applications.
For more details see the Single Cell tab above.
DNA/RNA quantification and QC
The core provides equipment and services for DNA/RNA quantification and integrity assessment using the following platforms:
- TapeStation
- Nanodrop
- Qubit
Other Instruments
The following instruments and equipment are available for use to local users.
- Sage Science BluePippin - Automated DNA size selection (100bp-80kb)
- Biorad CFX Opus 96 - RT-PCR machine
- Qiagen Tissue Ruptor 2 - Homogenization of tissue
- Covaris M220 Focused ultrasonicator - Nucleic acid size shearing
- PCR Machines
General services
- DNA/RNA extraction from a variety of source material including blood, buffy coat, saliva, fresh or frozen tissue, cultured cells, FFPE blocks, and microscope slides.