Equipment & Services
Next Generation Sequencing
Our facility uses Illumina (short read) and Pacific Biosciences (long-read) sequencing platforms. We operate an Illumina NextSeq 2000, NovaSeq X Plus and a Pacific Biosciences Vega.
We offer library preparation and sequencing services for PacBio and a range of Illumina sequencing applications.
For more details see the Illumina Seq tab above.
10X Genomics Single Cell Sequencing
Our 10X Chromium Connect and Chromium iX are now for single cell RNAseq applications.
For more details see the Single Cell tab above.
DNA/RNA quantification and QC
The core provides equipment and services for DNA/RNA quantification and integrity assessment using the following platforms:
- TapeStation
- Nanodrop
- Qubit
Other Instruments
- Sage Science BluePippin - Automated DNA size selection (100bp-80kb)
- Biorad CFX Opus 96 - RT-PCR machine
- Qiagen Tissue Ruptor 2 - Homogenization of tissue
- Covaris M220 Focused ultrasonicator - Nucleic acid size shearing
General services
- DNA/RNA extraction from a variety of source material including blood, buffy coat, saliva, fresh or frozen tissue, cultured cells, FFPE blocks, and microscope slides.