Equipment & Services

Next Generation Sequencing

Our facility uses Illumina and Pacific Biosciences (long-read) sequencing platforms. We operate a Pacific Biosciences Sequel II and Illumina NextSeq 2000.

We offer library preparation and sequencing services for PacBio long read DNA sequencing and a range of Illumina sequencing applications, including:

Exome Sequencing

Exome sequencing is a simple, efficient method for high-confidence calling of exonic variants. The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.

Starting from just 50ng of genomic DNA this kit uses transposase-based fragmentation and exome enrichment with biotinylated capture probes to capture coding exons covering 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Multiplexing up to 12 samples per flowcell can be achieved. As well as the raw sequence data we also supply filtered variants lists and basic bioinformatics.

Please contact us for pricing.

ChIP-Seq

Chromatin immunoprecipitation (ChIP) sequencing is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Following ChIP-Seq protocols, DNA-bound protein is immunoprecipitated using a specific antibody.  The bound DNA is the coprecipitated, purified, and sequenced. 

Through examining protein-nucleic acid interactions at a genome-wide scale, ChIP-Seq reveals insights into gene regulation events involved in various diseases and biological pathways.

miRNA-Seq

Small RNA sequencing is a technique to isolate and sequence small RNA species, such as miRNAs.  Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.

DNA-Seq

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, etc. Multiplex up to 384 samples. Ideal for small genomes and works for any species.

Total RNA-Seq

Whole-transcriptome analysis with total RNA sequencing defects coding plus multiple forms of non-coding RNA.

Total RNA-Seq can accurately measure transcript abundance and identify known and novel features of the transcriptome.

 

DNA/RNA quantification and QC

The core provides equipment and services for DNA/RNA quantification and integrity assessment using the following platforms:

  • Agilent TapeStation
  • Thermo Scientific Nanodrop 
  • Invitrogen Qubit 
  • Covaris size shearing

Other Instruments

  • Sage Science BluePippin automated DNA size selection (100bp-80kb)
  • Biorad CFX Opus96 RT-PCR machine
  • Qiagen Tissue Ruptor 2

General services

  • DNA/RNA extraction from a variety of source material including blood, saliva, tissue, cultured cells, FFPE blocks, and slides.