Equipment & Services
Next Generation Sequencing
Our facility uses Illumina (short read) and Pacific Biosciences (long-read) sequencing platforms. We operate an Illumina NextSeq 2000 and a Pacific Biosciences Sequel II.
We offer library preparation and sequencing services for PacBio and a range of Illumina sequencing applications, including:
10X Genomics Single Cell Sequencing
Our 10X Chromium Connect and Chromium iX are now available to the community for single cell RNAseq applications.
If you're new to single cell RNASeq, here are some introductory documents from 10X Genomics.
Single cell RNA-seq: An introductory overview and tools for getting started
Getting Started: Single Cell 3’ Gene Expression
Here are some resources from 10X Genomics to help you get your samples ready.
Cell Preparation for Single Cell Protocols
Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling
Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling
DNA/RNA quantification and QC
The core provides equipment and services for DNA/RNA quantification and integrity assessment using the following platforms:
- TapeStation
- Nanodrop
- Qubit
Other Instruments
- Sage Science BluePippin - Automated DNA size selection (100bp-80kb)
- Biorad CFX Opus 96 - RT-PCR machine
- Qiagen Tissue Ruptor 2 - Homogenization of tissue
- Covaris M220 Focused ultrasonicator - Nucleic acid size shearing
General services
- DNA/RNA extraction from a variety of source material including blood, buffy coat, saliva, fresh or frozen tissue, cultured cells, FFPE blocks, and microscope slides.