Equipment & Services

Next Generation Sequencing

Our facility uses Illumina (short read) and Pacific Biosciences (long-read) sequencing platforms. We operate an Illumina NextSeq 2000 and a Pacific Biosciences Sequel II.

We offer library preparation and sequencing services for PacBio and a range of Illumina sequencing applications, including:

Small Genome

Small genome sequencing (≤ 5 Mb) involves sequencing the entire genome of a bacterium, virus, or other microbe, and then comparing the sequence to a known reference. Sequencing small microbial genomes can be useful for food testing in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.

Exome Sequencing

Exome sequencing is a simple, efficient method for high-confidence calling of exonic variants. The Illumina Kit delivers an average of >85% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.

Starting from just 50ng of genomic DNA this kit uses transposase-based fragmentation and exome enrichment with biotinylated capture probes to capture coding exons covering 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). As well as the raw sequence data we also supply filtered variants lists and basic bioinformatics.

Please contact us for pricing.

RNA-Seq

Whole-transcriptome analysis with total RNA sequencing defects coding plus multiple forms of non-coding RNA.

Total RNA-Seq can accurately measure transcript abundance and identify known and novel features of the transcriptome.

Other

ChIP-Seq

Chromatin immunoprecipitation (ChIP) sequencing is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Following ChIP-Seq protocols, DNA-bound protein is immunoprecipitated using a specific antibody.  The bound DNA is the coprecipitated, purified, and sequenced. 

Through examining protein-nucleic acid interactions at a genome-wide scale, ChIP-Seq reveals insights into gene regulation events involved in various diseases and biological pathways.

ATAC-Seq

The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression.

miRNA-Seq

Small RNA sequencing is a technique to isolate and sequence small RNA species, such as miRNAs.  Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.

TSO500

TruSight Oncology 500 is a next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling of tumor samples. It supports identification of all relevant DNA and RNA variants implicated in various solid tumor types.

In addition, it accurately measures key current immuno-oncology biomarkers: microsatellite instability (MSI) and tumor mutational burden (TMB).

 

10X Genomics Single Cell Sequencing

Our 10X Chromium Connect and Chromium iX are now available to the community for single cell RNAseq applications.

If you're new to single cell RNASeq, here are some introductory documents from 10X Genomics.

Single cell RNA-seq: An introductory overview and tools for getting started

Getting Started: Single Cell 3’ Gene Expression

Here are some resources from 10X Genomics to help you get your samples ready.

Cell Preparation for Single Cell Protocols

Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling

Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling

Flow Cytometry Guidance for Single Cell Protocols

 

DNA/RNA quantification and QC

The core provides equipment and services for DNA/RNA quantification and integrity assessment using the following platforms:

  • TapeStation
  • Nanodrop 
  • Qubit 

Other Instruments

  • Sage Science BluePippin - Automated DNA size selection (100bp-80kb)
  • Biorad CFX Opus 96 - RT-PCR machine
  • Qiagen Tissue Ruptor 2 - Homogenization of tissue
  • Covaris M220 Focused ultrasonicator - Nucleic acid size shearing

General services

  • DNA/RNA extraction from a variety of source material including blood, buffy coat, saliva, fresh or frozen tissue, cultured cells, FFPE blocks, and microscope slides.