Biological Mass Spectrometry Core Facility

Recommended literature

Below is a selection of key recent publications and videos from the scientific community on mass spectrometry. These influential works help us to continuously enhance the services provided by the BMS Core Facility, while allowing you to delve deeper into mass spectrometry. Be sure to regularly visit this page, as our team is continuously adding new materials.

Peer-reviewed Research Articles

Daly et al. (2025) - Optimized workflow for in-depth phosphoproteomics

Original work:

Daly et al. (2025). An Optimized SP3 Sample Processing Workflow for In-Depth and Reproducible Phosphoproteomics. Journal of Proteome Research, 24 (8), 4300-4308. DOI: 10.1021/acs.jproteome.5c00220

Main insights:

Comparative study of the different cell lysis procedures, protein processing methods and phospho-site enrichment strategies available in the scientific literature. This work introduces an optimized sample processing workflow for phosphoproteomics by obtaining roughly 1.6-; 6- and 36-fold increases in singly, doubly and triply phosphorylated peptides, respectively, compared to previous studies.

Nickerson and Doucette (2020) - Optimized method for fast and quantitative protein precipitation

Original work:

Nickerson and Doucette (2020). Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry. Journal of Proteome research, 19 (5), 2035-2042. DOI: 10.1021/acs.jproteome.9b00867

Main insights:

Development of a quick and unbiased method (in regard to molecular weight, isoelectric point and hydrophobicity) to precipitate proteins in a complex mixture. While maximizing protein recovery, this method can be used to concentrate a diluted lysate or to change solvents.

Hughes et al. (2019) - Development of the SP3 beads for efficient protein clean-up

Original work:

Hughes et al. (2019). Single-pot, solid-phase-enhanced sample preparation for proteomics experiments. Nature protocols, 14:68-85. DOI: 10.1038/s41596-018-0082-x

Main insights:

Development of the SP3 beads technology that allows unbiased and robust protein purification prior proteomic analysis. SP3 beads are routinely used at the BMS Core Facility to process samples for mass spectrometry.

   

Scientific Videos

What's inside our mass spectrometers?

Have you ever wondered what the mass spectrometer responsible for your data acquisition is made of? These videos will show you the inside of the Orbitrap Fusion Lumos, the QTrap 5500 and the QExactive; the main instruments used at the BMS Core Facility.

Each video below offers a cross-sectional view of the selected instrument and animates the peptide processing workflow within the machine. Punctual annotations on crucial steps are also available.