Neale D. Ridgway

Education

• PhD, University of British Columbia

Academic Positions

• Department member since 1991

Research Topics

Cell Biology of Cholesterol and Lipids

Publications

1. S. R. Barger, S. Sepúlveda, H.Yang, M. Goudge, S. Lee, N. D. Ridgway, S. Bahmanyar (2026) Hierarchical membrane-chromatin tethering buffers nuclear envelope assembly against alterations in lipid flux. BioRxiv. [PubMed]
2. M. Tavasoli, M. Alkandari, G. Dorighello, M. McPhee, N.D. Ridgway, K. Isaac, S. Sokolenko, R. Maroofian, A. Shukla, M. S Zaki, H. Houlden, and C. R. McMaster (2026) Neurodevelopmental Disease-causing CHKA Variants Couple Phosphatidylcholine Synthesis to Oxidative Stress Damage and Disease Etiology. J. Biol. Chem. doi: 10.1016/j.jbc.2025.110983. [PubMed]
3. M. McPhee^, J. Salsman, A. A. Newman, N. Voutsina, A. H. Crosby, G.  Dellaire, and N.  D. Ridgway (2026) Membrane curvature elastic stress triggers recruitment of PML-II onto the inner nuclear membrane. Mol. Biol. Cell. 27; Ar10. [PubMed]
4. A. Wilson, N.D. Ridgway, C. McCormick (2025) Primary envelopment of Kaposi’s sarcoma-associated herpesvirus at the nucleoplasmic reticulum.  J. Virology. e00588-25. [PubMed] 
5. M. McPhee, G. Dellaire and N.D. Ridgway (2024) Mechanisms for assembly of the nucleoplasmic reticulum.  Cell Mol Life Sci. 81; 415. [PubMed]
6. J. Thompson, F-M. Boisvert, J. Salsman, G. Dellaire  and N.D. Ridgway (2024) The proximity interactome of PML I and II under fatty acid stress. FEBS lett.599; 682-699. [PubMed] 
7. M. McPhee, J. Lee, J. Salsman, M. Pinelli, F. Di Cara, K. Rosen^, G. Dellaire and N.D. Ridgway (2024) Nuclear lipid droplets in Caco2 cells originate from nascent precursors and in situ at the nuclear envelope. J. Lipid Res. 65, 100540. [PubMed]
8. J. Foster, G. Dellaire, S. Pelech, N.D. Ridgway (2024) Lipid- and phospho-regulation of CTP:phosphocholine cytidylyltransferase a association with nuclear lipid droplets. Mol. Biol. Cell. Ar33, 1-14. [PubMed]
9. G.G. Dorighello, M. McPhee, K. Halliday, G. Dellaire and N.D. Ridgway (2023) Differential contributions of choline/ethanolamine phosphotransferase 1 (CEPT1) and choline phosphotransferase 1 (CHPT1) to phosphatidylcholine homeostasis and lipid droplet biogenesis. J Biol. Chem. 299(4) 104578. [PubMed] 
10. G.G. Dorighello, L.H.P. Assis, T. Rentz, J. Morari, M.F.M. Santana, M. Passarelli, N.D. Ridgway, A.E. Vercesi and H.C.F. Oliveira (2022) Novel role of CETP in macrophages: reduction of mitochondrial oxidants production and modulation of cell immune-metabolic profile. Antioxidants 11: 1734. [PubMed]
11. M.J. McPhee, J. Salsman, J. Foster, J. Thompson, S. Mathavarajah, G. Dellaire and N. D. Ridgway (2022) Running 'LAPS' around nLD: Nuclear lipid droplet form and function. Frontiers Cell Dev. Biol. 10:837406. [PubMed]
12. Y.X.I Tu, A. M. Sydor, E. Coyaud, E. M. N. Laurent, D. Dyer, N. Mellouk, J. St-Germain, R. M. Vernon, J. D. Forman-Kay, T. Li, R. Hua, K. Zhao, N.D. Ridgway, P. K. Kim, , B. Raught, and J. H. Brumell (2021) Global proximity interactome analysis of the human macroautophagy pathway.  Autophagy 15: 1-15. [PubMed]
13. W.P.D.W. Thilakarathna, H.P.V. Rupasinghe and N.D. Ridgway (2021) Mechanisms by which probiotic bacteria attenuate the risk of hepatocellular carcinoma. Int. J. Mol. Sci. 22; 2606-2615. [PubMed]
14. Kexin Zhao, Jason R. Foster and N.D. Ridgway (2020) ORP1 variants have opposing cholesterol transport activities from the endolysosomes. Mol. Biol. Cell. 31; 793-802. [PubMed]
    
15. L. Yue*, M. J. McPhee*, K. Gonzalez, M. Charman, J. Lee, J. Thompson, D. Winkler, R.B. Cornell, S. Pelech and N.D. Ridgway (2020) Differential dephosphorylation of CTP:phosphocholine cytidylyltransferase upon translocation to nuclear membranes and lipid droplets. Mol. Biol. Cell. 31; 1047-1059. [PubMed]
    
16. J. Lee, J. Salsman, J. Foster, G. Dellaire and N.D. Ridgway (2020) Lipid-associated PML structures (LAPS) assemble nuclear lipid droplets containing CCTα and Lipin1. Life Sci Alliance. 3:8 [PubMed]
    
17. A. Pietrangelo and N.D. Ridgway, (2019) Phosphorylation of a serine/proline-rich motif in oxysterol binding protein-related protein 4L (ORP4L) regulates cholesterol and vimentin binding. Plos One 14:e0214768 [PubMed]
18. J. Lee and N.D. Ridgway, (2019) Substrate channeling in the glycerol-3-phosphate pathway regulates the synthesis, storage and secretion of glycerolipids Biochim Biophys Acta : [PubMed]
19. A. Pietrangelo and N.D. Ridgway, (2018) Bridging the molecular and biological functions of the oxysterol binding protein family. Cell Mol. Life Sci. :1-20 [PubMed] [Article]
20. J. Huang, C.J. Mousley, L. Dacquay, Nairita Maitra, G. Drin, C. He, N.D. Ridgway, M.Kennedy, B.K. Kennedy, K. Baetz, M. Polymenis, and V.A. Bankaitis, (2018) A Lipid Transfer Protein Signaling Axis Exerts Dual Control of Cell-Cycle and Membrane Trafficking Systems Dev. Cell 44:378-391 [PubMed]
21. K. Zhao, A. van der Spoel, C. Castiglioni, S. Gale, D.S. Ory and N.D. Ridgway , (2018) 19q13.12 microdeletion syndrome fibroblasts display abnormal storage of cholesterol and sphingolipids in the endo-lysosomal system Biochem. Biophys. Acta Mol. Mech. Dis 1864:2108-2118 [PubMed]
22. P. Mukherjee, H. Madarati, N.D. Ridgway, and J. Atkinson , (2018) Lipid and membrane recognition by the oxysterol binding protein and its phosphomimetic mutant using dual polarization interferometry. Biochem. Biophys. Acta Biomembranes 1860:2356-2355 [PubMed]
23. N.D. Ridgway and K. Zhao , (2018) Cholesterol transfer at endosomal-organelle membrane contact sites Curr. Opin. Lipidology 29:212-217 [PubMed]
24. A. Pietrangelo and N.D. Ridgway , (2018) Golgi localization of oxysterol binding protein-related protein 4L (ORP4L) is regulated by ligand binding J. Cell Sci. 131 (14):jcs215335 [PubMed]
25. Ridgway, N.D. , (2018) How CCTα puts a leash on phospholipid synthesis J. Biol. Chem. 293:7085-7086 [PubMed]
26. A. Goto, M. Charman, and N.D. Ridgway, (2018) Protein kinase D1 and oxysterol binding protein form a regulatory complex independent of phosphorylation Traffic 19:854-866 [PubMed]
27. Lee, J. and Ridgway N.D., (2018) Phosphatidylcholine synthesis regulates triglyceride storage and chylomicron secretion by Caco2 cells. J. Lipid Res. 59:1940-1950 [PubMed]
28. Charman, M., Goto, A. N., Ridgway, N.D. , (2017) Oxysterol binding protein recruitment and activity at the ER-Golgi interface are independent of Sac1 Traffic 18:519-529 [PubMed]
29. Zhao, K., Ridgway N.D. , (2017) ORP1L regulates cholesterol egress from the endo-lysosomal system Cell Reports 19:1807-1809 [PubMed]
30. Goto, A., Charman, M. and Ridgway N.D., (2016) Oxysterol Binding Protein Activation at Endoplasmic Reticulum-Golgi Contact Sites Reorganizes Phosphatidylinositol 4-Phosphate Pools J. Biol. Chem. 219:1336-1347 [PubMed]
31. Ridgway, N.D., (2016) Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine Methods Mol. Biol. 1376:195-202 [PubMed]
32. Maekawa, M., Lee, M., Wei, K., Ridgway, N.D., and Fairn, G.D. , (2016) Staurosporines decrease ORMDL proteins and enhance sphingomyelin synthesis resulting in depletion of plasmalemmal phosphatidylserine Sci Reports 6:35726 [PubMed]
33. Aitchison, A.J., Arsenault, D.J., and Ridgway, N., (2015) Nuclear-localized CTP: phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis Mol. Biol. Cell 26(16):2927-2938 [PubMed] [Article]
34. Ridgway , N.D. and McLeod, R.S., (2015) Biochemistry of Lipids, Lipoproteins and Membranes Editors: Ridgway and McLeod : [Article]
35. Cornell, R.B. and Ridgway N.D., (2015) CTP: phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis. Prog. Lipid Res. 59:147-171 [PubMed]
36. Liu, X. and Ridgway, N.D., (2014) Characterization of the sterol and phosphatidylinositol 4-phosphate binding properties of Golgi-associated OSBP-related protein 9 (ORP9) Plos One 9 (9):e108368: [PubMed]
37. Charman, M., Colbourne, T.R., Pietrangelo, A., Kreplak, L., Ridgway, N.D., (2014) Oxysterol-binding protein (OSBP)-related protein 4 (ORP4) is essential for cell proliferation and survival J.Biol.Chem 289:15705-15804 [PubMed]
38. Park, IW., Ndjomou, J., Wen, Y., Liu Z, Ridgway, ND., Kao, CC., He, JJ., (2013) Inhibition of HCV Replication by Oxysterol-Binding Protein-Related Protein 4 (ORP4) through Interaction with HCV NS5B and Alteration of Lipid Droplet Formation. PloS One 17:e75648 [PubMed]
39. Arsenault DJ, Yoo BH, Rosen KV, Ridgway ND, (2013) ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells. J. Biol. Chem. 288:633-43 [PubMed]
40. Lagace, T.A. and Ridgway, N.D. , (2013) The role of phospholipids in the biological activity of the endoplasmic reticulum Biochim. Biophys. ACTA 1833:2499-2510 [PubMed]
41. Morton, C.C., Aitchison, A.J., Karsten, G. and and Ridgway, N.D., (2013) A mechanism for suppression of the CDP-choline pathway during apoptosis J. Lipid Res. 54:3373-3381 [PubMed]
42. Ridgway, N.D., (2012) The role of phosphatidylcholine and choline metabolites in cell proliferation and survival Critical Reviews in Biochemistry and Molecular Biology 48:20-38 [PubMed]
43. A. Goto, X. Liu, C. Robinson and N. Ridgway, (2012) Multi-Site Phosphorylation of Oxysterol Binding Protein (OSBP) Regulates Sterol Binding and Activation of Sphingomyelin Synthesis Mol. Biol. Cel 23:3624-3635 [PubMed]